Journal: Biosafety and Health

Article Title: Evaluating viral inactivation in the liquid waste stream from a viral total nucleic acid extraction kit for safe disposal

doi: 10.1016/j.bsheal.2025.09.001

Figure Lengend Snippet: Log 10 reduction of MS2 infectivity over time in liquid waste. The horizontal axis represents the incubation time (hours), and the vertical axis represents the log 10 reduction in viral infectivity. Blue circles correspond to individual replicate measurements. Red crosses indicate the empirically-derived average log 10 reduction at each time point. The red solid curve represents the exponential fit of the data using a one-phase decay model, described by the equation: Log 10 reduction = −4.63 × e −0.26 × time + 8.50 ( R 2 > 0.99). The pink shaded area represents the 95 % CI of the fitted curve. The horizontal dashed purple line indicates the 6-log 10 reduction threshold, while the vertical dashed purple lines mark the estimated mean time point (2.41 h) and the 95 % CI (1.34 to 4.05 h) of when the model predicts the 6-log 10 reduction is achieved. Abbreviation: CI, confidence interval.

Article Snippet: The bacteriophage MS2 (Deutsche Sammlung von Mikroorganismen und Zellkulturen [DSMZ] 13,767 strain) was propagated using Escherichia coli ( E. coli ) (DSMZ 5,695 strain) as the host organism.

Techniques: Infection, Incubation, Derivative Assay